Figure 4.
cdc13-1 ira1Δ ira2Δ has delayed recovery kinetics. α-factor arrested cultures were raised to 32° and released from the G1 arrest by micromanipulation onto 32° YPD agar. After three hours at 32°, plates were returned to 21° (T = 0 hours recovery). Colonies were scored at the indicated times and classified as visible to the naked eye (Recovered), microscopically visible with more than 2 cells (Microscopic) or as single large-budded cells (2-cell). Values are mean ± standard error of three assays. (A) cdc13-1, (B) cdc13-1 ira1Δ ira2Δ.