Figure 5.

Single-cell recovery assays reveal signaling elements required for the ira1Δ ira2Δ recovery defect. (A and B) Strains were treated as in Figure 4. Colonies were scored after 72 hours of recovery. (C) Strains were treated as in Figure 4 except cultures were in synthetic complete media lacking leucine (C-Leu) to maintain CDC20 plasmids. Values are mean ± standard error of at least three assays.