Table 1.
Strains and constructs used in this study
| Name | Genotype | Source |
| Y300 | MATa ade2-1 trp1-1 ura3-1 leu2-3,112 his 3-11,15 can1-1001 | Allen, et al. 1994 |
| Y816 | As Y300 cdc13-1 | Searle, et al. 2004 |
| Y818 | As Y300 cdc13-1 chk1Δ::HIS3 | Sanchez, et al. 1999 |
| Y831 | As Y300 cdc13-1 rad53-21 LEU2 HIS3 | Sanchez, et al. 1999 |
| YMW197 | As Y300 ira1Δ::URA3 | This study |
| YMW152 | As Y300 ira2Δ::URA3 | This study |
| YMW208 | As Y300 ira1Δ::URA3 ira2Δ::URA3 | This study |
| YMW198 | As Y816 ira1Δ::URA3 | This study |
| YMW142 | As Y816 ira2Δ::URA3 | This study |
| YMW199 | As Y816 ira1Δ::URA3 ira2Δ::URA3 | This study |
| YMW203 | cdc13-1 chk1Δ::HIS3 ira1Δ::URA3 ira2Δ::URA3 | This study |
| YMW461 | cdc13-1 ira1Δ::URA3 ira2Δ::URA3 rad53-21 | This study |
| YMW544 | cdc13-1 pds1Δ::LEU2 | This study |
| YMW545 | cdc13-1 ira1Δ::URA3 ira2Δ::URA3 pds1Δ::LEU2 | This study |
| YMW257 | cdc13-1 tpk2Δ::KAN | This study |
| YMW512 | cdc13-1 ira1Δ::URA3 ira2Δ::URA3 tpk2Δ::KAN | This study |
| YMW534 | cdc13-1 [pMW034] | This study |
| YMW535 | cdc13-1 [pMW036] | This study |
| YMW536 | cdc13-1 ira1Δ::URA3 ira2Δ::URA3 [pMW034] | This study |
| YMW537 | cdc13-1 ira1Δ::URA3 ira2Δ::URA3 [pMW036] | This study |
| YMW446 | cdc13-1/CDC13 ira1Δ::URA3/IRA1 ira2Δ::URA3/IRA2 a/α | This study |
| Name | Description | Source |
| pJS3 | HA-CDC20 in YCplac33, CEN URA3 | Searle, et al. 2004 |
| pJS6 | HA-CDC20 S52A S88A in YCplac33 CEN URA3 | Searle, et al. 2004 |
| pMW034 | HA-CDC20 in pRS415 (SmaI-SpeI fragment from pJS3) | This study |
| pMW036 | HA-CDC20 S52A S88A in pRS415 (SmaI-SpeI fragment from pJS6) | This study |
1
All strains are mating type a unless noted.