Figure 1.

p53- and -Hdm2 binding domains of HdmX are essential for blocking oncogenic Ras induced premature senescence. IMR90 cells were transduced at an MOI of 5 with the indicated lentivirus: (1) mock infection (Mock), (2) H-Ras (Ras), (3) GFP, (4) Ras + GFP, (5) Ras + HdmX, (6) Ras + Hdm2, (7) Ras + HdmXG57A (XG57A) or (8) Ras + HdmXC437G (XC437G), followed by incubation in complete media for 7 days at which time β-galactosidase staining was completed. (A) Histogram represents average ± SeM of % beta-galactosidase positive cells normalized to transduction efficiency (based on GFP). over 100 cells were counted per treatment and each infection was repeated in biological triplicate. (B) Representative images for infections as described in (A) are shown. Bright-field or fluorescence images were taken at same exposure time and 100X magnification. (C) Western blot demonstrating expression of the indicated proteins.