Figure 3.

Loss of HdmX induces senescence in LNCaps. (A) LNCap cells were transduced with no virus (Mock), GFP, shLacZ (shL), HdmX, HdmX-Rescue (X/R), shHdmX (shX), shL + X/R, shX + X/R or shX + GFp, selected for 7 days and subjected to western blotting using the indicated antibodies. (B) Average % β-galactosidase positive cells normalized to transduction efficiency (based on GFP) ±SEM is reported where >100 cells were counted per treatment (same as in A) in three biological replicates, except Mock, which is in duplicate.