PDI from C. textile venom duct. (A) SDS/PAGE of extracts from different parts of a venom duct. Lanes 1–4 represent equal fragments from distal to proximal parts of the venom duct. Lane 5 is a bovine PDI. The ≈55-kDa band from lane 3 was extracted, and its N-terminal sequence was determined (see text). (B) Structures of PDI-1 and PDI-2 from C. textile. The signal sequence, thioredoxin active sites (Trx), and the ER retention signal are marked. Domains a, b, b′,a′, and c are based on the assignments for human PDI (9, 10). The shaded residues represent differences between PDI-1 and PDI-2.