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Transcriptional sequencing with thio-analogs of UTP. Sequencing reactions of the template 1w1 (15) with intact SP6 RNA polymerase were carried out using (A) UTP, (B) S4UTP (4sU) or (C) UTPαS (αsU) and the other normal rNTPs. Multi-round transcription reactions of HindIII-digested 1w1 were run in parallel to the left of each panel. See Figure 1 legend.
