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. 2011 Feb;162(3):733–748. doi: 10.1111/j.1476-5381.2010.01082.x

Figure 7.

Figure 7

The role of tyrosine 3.32 of GPR35 in ligand function. Receptor-β-arrestin-2 interaction BRET assays were performed in response to varying concentrations of zaprinast (A, C) or kynurenic acid (B) following co-transfection of FLAG-rGPR35-eYFP, FLAG-Tyr3.32Ala rGPR35-eYFP or FLAG-Tyr3.32Leu rGPR35-eYFP (A, B) of the equivalent forms of human GPR35 (C). Effects of mutation of Tyr 3.32 on the response to 10−4 M compound 10 was also studied (D). Total expressed levels, as monitored by eYFP fluorescence (E), of the various forms of GPR35 were unaltered by these mutations, as was cell surface delivery monitored by anti-FLAG elisa (F). (G) The amino acid sequence of a section of transmembrane domain III from both human and rat GPR35 is shown with Tyr3.32 highlighted.