ToF-SIMS images showing the effect of cholesterol on the lateral distribution of miniB and PLs in BLES LB films. BLES supplemented with 20 mol% 15N-miniB and 20 mol% d7-cholesterol was compressed to a surface pressure of 30 mN/m and transferred onto gold-coated mica by conventional Langmuir–Blodgett technique. A–E were collected in positive and negative ion modes and show the distribution of M2 (deuterium) specific to d7-cholesterol, M45 (
) specific to 15N-miniB, M255 (palmitate), M722 (DPPG−) and M747 (POPG−), respectively. A1–E1 were obtained by subjecting A–E to a smoothing function available as part of the ION-TOF software to improve contrast. All images are 50×50 μm2, 256×256 pixels with 1 shot/pixel.