Table 1. Sequences of amplification primers and Molecular Beacon probes.
Primers |
Sequence |
RS40 |
5′-TTTTCCCACCCTTAGGCTG-3′ |
RS42 |
5′-CTCACTCAGTGTGGCAAAG-3′ |
47ex (8259–8281) |
5′-TTACCCTATAGCACCCCCTCTAC-3′ |
51ex (8396–8371) |
5′-TGGGCCATACGGTAGTATTTAGTTGG-3′ |
Molecular Beacons |
|
WT-594 (8334–8353) |
TET-5′-CCAGCG GATTAAGAGAACCAACACCT CGCTGG-3′-DABCYL |
MT-593 (8334–8353) |
FAM-5′-CCAGCG GATTAAGAGAGCCAACACCT CGCTGG-3′-DABCYL |
BG-736 |
FAM-5′-CCAGCG CTGTCCACTCCTGATGCTGTTAT CGCTGG-3′-DABCYL |
Primer pair |
Predicted product size |
RS40/RS42 |
198 bp |
47EX/51EX | 138 bp |
The underlined parts of Molecular Beacon sequences represent the stem and italic parts the loop sequences.