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The recombination consists of two events. 1. The integration of the shuttle vector pKOV-Kan into the BAC and 2. the resolution of the co-integrant into either the original or the mutant BAC. The position of primers used for PCR are shown. EcoRI sites on the BAC are indicated by vertical lines. EcoRI sites on the pKOV-Kan are indicated by arrows. E, EcoRI; m, mutant; n, normal nucleotide.
