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Effect of MMP-12 inhibition on brachiocephalic atherosclerotic plaque elastinolytic activity. Elastinolytic in situ zymography of brachiocephalic artery lesions with incubation buffer alone (A) or plus 100 nmol/L RXP470.1 (B), 20 mmol/L EDTA (C), or 1μmol/L BB94 (D). Green fluorescence represents elastinolytic activity. Scale bar in A represents 50 μm and is applicable to all panels.
