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IC transformation. Time course of IC degradation by purified laccase from B. pumilus monitored spectroscopically at λ = 650 nm over a total of 48 h at 37°C. Reactions were conducted at pH 6.5 and 7.8, in the presence or absence of ACS. (A) 0.25 mM IC and ACS added at a ratio of 1:100. (B) 1 mM IC and ACS added at a ratio 1:100. (C) 0.25 mM IC and ACS added at a ratio of 1:1000. (D) 1 mM IC and ACS added at a ratio of 1:1000.
