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. 2011 Feb 7;8:7. doi: 10.1186/1743-8977-8-7

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Copyright ©2011 Pascolo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Soft X-ray microscopy and XRF analyses of an asbestos body in human lung tissue. Panel A: visible light image of the tissue with the asbestos body. Panels B and C: absorption and phase contrast images respectively acquired at a photon energy of 2018 eV (25 × 40 μm²size, 30 ms dwell time, 415 nm spot size). Panels Fe, Si and Mg: elemental distribution (25 × 35 μm²size, 30 s/pixel dwell time, 1 μm spot size) among the area of panels B and C, acquired at 2 keV, (Si and Mg), and at 1,3 keV (Fe). Panel D and E: XRF spectra collected in the points a and b (see panel B) at 1.3 keV (D) and at 2 keV (E) with 180 s acquisition time).