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. 2011 Feb 8;11:2. doi: 10.1186/1475-2867-11-2

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Copyright ©2011 Somji et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MT-3 promoter (region 1) associated histone modifications in the cell lines after treatment with MS-275. A-D. ChIP-qPCR of acetyl H4, trimethy H3K4, trimethyl H3K9 and trimethyl H3K27 at the MT-3 promoter using primers for region 1. The amplification value of the immunoprecipitated DNA was normalized to percentage of input (non-precipitated DNA). The determinations were performed in triplicates and the results shown are the mean ± SE.*Statistically significant compared to untreated control cells. E. Ethidium bromide stained gel showing the amplification of the histone modifications using semiquantitative PCR.