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Sequences and PCR conditions for primers
| Promoter regions | Upper primer | Lower primer | Product size (bp) | Annealing temperature °C |
|---|---|---|---|---|
| MRE A & B | AAAGAGCGGGCGCGGTGC | GACGCGCGGCTTGGCTAGTGG | 111 | 75 |
| MRE C | GGCCCCGGCAGTGCACA | GCGCACGCACTGCATCTGTCG | 55 | 75 |
| MRE E, F, G | ATGGTACGTGCGCGCTTCC | CATCCGCGTGCACGACCCACT | 124 | 70 |
| Region 1 | GAACAGATCTGGCGTCCTG | GCGCACGTACCATCTCCGA | 111 | 63 |
| Region 2 | GTCGGGCTCATCGTGA | ATTCTCCAGGACGCCAGAT | 77 | 61 |
