Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Every cloning step relies on three elements that are different from one level to the next: antibiotic selectable marker, type IIS enzyme(s), and color selectable marker. Cloning after level 2i-1 requires the simultaneous use of two type IIS enzymes: BpiI/BsaI or BpiI/Esp3I.
