Table 1. Summary of LFB staining for presence of demyelination in ON, SC, and brain of SCID mice following adoptive transfer.a .
| Number of mice with CNS demyelination following infection withc | ||
| Adoptive transfer b | HSV-IL-2 | WT KOS |
| nCD4+CD25+ | 0/5 (0%) | 0/5 (0%) |
| nCD4+CD25− | 5/5 (100%) | 0/5 (0%) |
| nCD8+CD25+ | 5/5 (100%) | 0/5 (0%) |
| nCD8+CD25− | 5/5 (100%) | 0/5 (0%) |
| effCD8+CD25+ | 5/5 (100%) | 5/5 (100%) |
| effCD8+CD25− | 5/5 (100%) | 5/5 (100%) |
| effCD4+CD25+ | 5/5 (100%) | 0/5 (0%) |
| effCD4+CD25− | 5/5 (100%) | 5/5 (100%) |
| Media | 0/5 (0%) | 0/5 (0%) |
a
Presence of demyelination in ON, SC, and brain of 5 recipient mice per group were assessed on day 14 PI.
b
Naive T cells (nCD8+CD25+, nCD8+CD25−, nCD4+CD25+, and nCD4+CD25−) were isolated from naive mice, while the effector T cells (effCD8+CD25+, effCD8+CD25−, effCD4+CD25+, and effCD4+CD25−) were isolated from mice infected with HSV-IL-2 on day 5 PI. Each SCID mice received 2×105 cells in 300 µl of tissue culture media or tissue culture media only.
c
Differences between group with CNS demyelination versus group with no CNS demyelination were statistically significant (p = 0.008, n = 5, Fisher exact test).