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Construction of luciferase-recombinant CMV viruses and confirmation of luciferase orientation by PCR. 1(A): Construction of luciferase-recombinant Towne, insertion of promoter and luciferase reporter between US9 and US10. Appropriate restriction sites, the primers used for verification and the expected size of PCR products are depicted. 1(B): PCR of pp28- and POL-luciferase constructs. Lane 1-4: primers 1+ 4, lane 5-8: primers 1+2, lane 9-12: primer 3+4.
