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Luciferase expression and real-time PCR. HFF were infected with pp28-luciferase and treated with either GCV or dimer sulfone carbamate. Luciferase activity was determined in cell lysates of infected-treated cells and infected non-treated cells. DNA copy number was determined by real-time PCR in supernatants of infected-treated cells and infected non-treated cells 6 days post infection.
