Table 1.
Oligonucleotides for assembly of Circovirus Pcap cores and PCV-1 CLE
| PCV-1 CLE | CLE-Fwd | 5'-CTAGCTAGTGGGCCCGCCCGGTTGTGGGAAGAGCCAGTGG GCCCGTAATTTTG - 3' |
|---|---|---|
| CLE-Rev | 5'-CTAGCAAAATTACGGGCCCACTGGCTCTTCCCACAACCGG GCGGGCCCACTAG - 3' | |
| PCV-1 Pcap core | PCV-1 Fwd-A | 5'-CTAGTCCTAGGCTGAACTTTTGAAAGTGAGCGGGAAGATGCAGCAGCGTG - 3' |
| PCV-1 Fwd-B | 5'-ATTGGAAGACAGCTGTACACGTCATAGTGGCTAGCA-3' | |
| PCV-1 Rev-C | 5'-CTAGTGCTAGCCACTATGACGTGTACAGCTGTCTTCCAATCACGCTGCTGCATCTTCCCG-3' | |
| PCV-1/PCV-2 Rev-D | 5'-CTCACTTTCAAAAGTTCAGCCTAGGA - 3' | |
| PCV-2 Pcap core | PCV-2 Fwd-P | 5'-CTAGTCCTAGGCTGAACTTTTGAAAGTGAGCGGGAAAATGCAGAAGCGTG-3' |
| PCV-2 Fwd-Q | 5'-ATTGGAAGACTAATGTACACGTCATTGTGGCTAGCA-3' | |
| PCV-2 Rev-R | 5'-CTAGTGCTAGCCACAATGACGTGTACATTAGTCTTCCAATCACGCTTCTGCATTTTCCCG-3' | |
| BFDV Pcap core | BFDV Fwd-W | 5'-CTAGTCCTAGGGTTCCCAGATATCTACGTCAGGCATGGGCGGGGCTTAC-3' |
| BFDV Fwd-X | 5'-ATAATCTCTCGCTAGCTAGCAGCTAGCA-3' | |
| BFDV Rev-Y | 5'-CTAGTGCTAGCTGCTAGCTAGCGAGAGATTATGTAAGCCCCGCCCATGC-3' | |
| BFDV Rev-Z | 5'-CTGACGTAGATATCTGGGAACCCTAGGA-3 | |