Figure 7. A mixed immunization regime combining both clades (B and BF) induced a broad immune response.

A) Immunization scheme describing viral and DNA doses applied. B) Ten days after the boost immunization, T cell response induced after the mixed immunization was analyzed against the different B and BF peptides targeted after the single-clade immunizations. Peptides were grouped according to its localization within each protein region; #: indicates that positive responses were found in one of two experiments. C) Ten days after the boost, splenocytes from mice immunized as its is depicted in A or in Figure 2A were stimulated with gp120 Bal (1 µg/ml) during 72 hrs and afterwards IFN-γ levels in the supernatant were quantified.