Table 2. Weight and morphological features of WAT in 25 d and 6 m old animals.
Males | Females | |||||
C | CR | C | CR | |||
25 d old | ||||||
Body weight (g) | 63.2±3.3 | 59.3±2.0 | 60.4±1.8 | 55.7±2.8 | ||
iWAT | ||||||
Weight (mg) | 632±69 | 684±49 | 689±98 | 739±53 | ||
Adipocyte diameter (µm) | 33.4±1.9 | 32.0±0.8 | 34.3±1.3 | 30.4±1.1 | ||
DNA (µg/g wet tissue) | 954±94 | 837±73 | 857±121 | 897±62 | ||
Total DNA content (mg) | 573±44 | 565±53 | 551±60 | 670±83 | ||
rpWAT | ||||||
Weight (mg) | 113±12 | 95.7±7.8 | 95.4±16.5 | 92.2±18.4 | ||
Adipocyte diameter (µm) | 30.9±1.9 | 32.6±1.0 | 34.0±1.8 | 33.0±2.8 | ||
DNA (µg/g wet tissue) | 682±40 | 719±69 | 728±126 | 634±73 | ||
Total DNA content (mg) | 75.4±6.1 | 68.9±9.9 | 74.1±24.7 | 59.6±15.1 | ||
6 m old | ||||||
Body weight (g) | 487±9 | 548±21* | 263±14 | 274±5 | ||
iWAT | ||||||
Weight (g) | 11.7±1.1 | 16.3±1.6* | 3.54±0.49 | 3.83±0.38 | ||
Adipocyte diameter (µm) | 71.6±2.6 | 71.8±3.3 | 54.2±2.6 | 58.4±3.0 | ||
DNA (µg/g wet tissue) | 188±30 | 176±9 | 301±48 | 286±60 | ||
Total DNA content (mg) | 1981±279 | 2825±221* | 989±126 | 1023±210 | ||
rpWAT | ||||||
Weight (g) | 15.3±1.1 | 19.0±1.7 | 2.96±0.34 | 4.43±0.61 | ||
Adipocyte diameter (µm) | 79.7±4.3 | 95.5±3.9* | 76.6±4.1 | 75.6±3.5 | ||
DNA (µg/g wet tissue) | 123±3 | 97.6±11.3 | 73.7±5.1 | 70.0±11.1 | ||
Total DNA content (mg) | 1896±148 | 1823±236 | 223±36 | 285±35 |
Body weight and weight, adipocyte diameter, DNA expressed per gram of wet tissue and total DNA content of inguinal and retroperitoneal white adipose tissue depots (iWAT and rpWAT respectively), in 25 d and 6 m old male and female offspring of controls (C) and 20% caloric restricted dams during gestation (CR). Each group is made up of animals from at least four different litters. Data are mean ± s.e.m.
*Different from their respective C group (p<0.05; Student's t -test).