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Depth sensitivity for functionally activated columns of four different cross-sectional areas: (a) 10 × 10, (b) 50 × 50, (c) 100 × 100, and (d) 200 × 200 μm2, respectively, for fluorescence imaging with the nonuniform distribution of the fluorescent targets. In (a)–(d), NA is fixed at 0.2 and the focal plane depth is varied among 100 (circle), 300 (star), and 500 (triangle) μm.
