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Depth sensitivity for functionally activated columns of four different cross-sectional areas: (a) 10 × 10, (b) 50 × 50, (c) 100 × 100, and (d) 200 × 200 μm2, respectively, for absorption imaging. In (a)–(d) NA is fixed at 0.2 and the focal plane depth is varied among 100 (blue), 300 (circle), and 500 (triangle) μm.
