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Depth sensitivity for functionally activated columns of four different cross-sectional areas: (a) 10 × 10, (b) 50 × 50, (c) 100 × 100, and (d) 200 × 200 μm2, respectively, for absorption imaging. In (a)–(d), the focal plane depth NA is fixed at 300 μm and NA is varied among 0.1 (circle), 0.2 (star), and 0.4 (triangle).
