Figure 7. TAMs control the replication of Brucella abortus.

A. Cells were infected with B. abortus for 45 minutes at 37°C at a MOI 100∶1 and medium containing 100 µg/ml gentamicin was added for 1 hour to kill extracellular bacteria. Intracellular bacteria were detected by immunofluorescence analysis as described in Materials and Methods. B. Intracellular growth curve of B. abortus. Cells were infected 45 minutes at 37°C at a MOI 100∶1 and medium containing 100 µg/ml gentamicin was added for 1 h to kill extracellular bacteria (0 h). Cells were washed and RPMI supplemented with 5 µg/ml gentamicin was added. At the indicated periods of time, cells were lysed and the CFU quantified as described in Figure 3A. A representative experiment is shown. C. Summary of growth curves of B. abortus. The data are presented as percentage of the number of CFU in TAMs relative to the number of CFU in control monocytes (SEM of three independent experiments).