Figure 5. Thiostrepton with intact B ring potentiates chromatin condensation in vitro detection kit.

Treatment with combination of thiostrepton/bortezomib results in appearance of sub-G₀ peak which reduces the fluorescence intensity in HCT-116 cells as opposed to untreated control cells due to fragmentation of chromatin. The shift of the FI was comparable to the positive control provided in the kit (Staurosporine, data not shown). Thiostrepton-methyl ester with open B ring does not affect the fluorescence intensity as opposed to untreated (control) cells.