Table 1.
Action of Cl− channel inhibitors
| CaCCinh-A01 | GlyH-101 | NPPB | ||
|---|---|---|---|---|
| 30μM (n = 4) | 10μM (n = 4) | 10μM (n = 3) | ||
| adrenaline: | 1st peak | ↑ +101.9±21.0 {0.017} | ↑ +48.3±9.1 {0.013} | ↔ −15.8±61.5 {0.82} |
| 2nd peak | ↑ +147.6±18.4 {0.004} | ↑ +169.3±28.6{0.010} | ↔ +11.5±27.5 {0.72} | |
| plateau | ↓ +118.3±7.5 {0.001} | ↓ * | ↓ * | |
|
| ||||
| PGE2: | 1st peak | ↑ +200.2±38.5 {0.014} | ↑ +118.0±34.1{0.041} | ↔ +13.1±86.5 {0.89} |
| 2nd peak | ↑ +205.5±42.9 {0.017} | ↑ +290.7±16.3{0.001} | ↑ +95.6±21.1 {0.045} | |
| plateau | ↑ +203.6±18.1 {0.002} | ↔ +71.8±25.4 {0.07} | ↑ +58.7±3.2 {0.003} | |
|
| ||||
| CCh/PGE2: | 1st peak | ↑ +191.3±40.8 {0.018} | ↓ −194.2±14.9{0.001} | ↔ +75.7±31.1 {0.14} |
| 2nd peak | ↔ +1.0±69.0 {0.98} | ↓ −345.9±47.1{0.005} | ↓ −98.3±15.7 {0.025} | |
| plateau | ↔ −29.5±54.9 {0.63} | ↓ −221.2±9.8 {0.001} | ↓ −150.4±28.3 {0.034} | |
Secretagog activated Isc were compared in the presence and absence of inhibitors (ΔISC = inhibitor – control; |μA/cm2) for adrenaline (Fig 1A), PGE2 (Fig 3B), and CCh/PGE2 (Fig 3C). Significant increases in activation (stimulation, ↑) and decreases in activation (inhibition, ↓) are indicated {P values}.
*
Data in Fig 1C.