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. Author manuscript; available in PMC: 2012 Feb 15.

Published in final edited form as: Clin Cancer Res. 2010 Nov 18;17(4):771–782. doi: 10.1158/1078-0432.CCR-10-2444

CNTs enhance CpG uptake in vitro. Primary bone marrow-derived monocytes (BMM) from wt or CX3CR1^GFP mice or GL261 gliomas were incubated with PEG-functionalized single-walled CNTs conjugated to Cy5.5-labeled sCpG (CNT-sCpG^5.5, 2.5 µg CNT- 5µg sCpG^5.5/ml) or free sCpG^5.5 (5µg/ml) and sCpG^5.5 uptake was visualized by fluorescent microscopy and quantified by flow cytometry. A, dot plot demonstrating CNT-sCpG uptake by BMM to be more efficient than free sCpG^5.5. B, CpG^5.5 signal was stronger in BMM.gfp cells incubated with CNT-sCpG and was mostly confined to cytoplasmic compartments. C, histogram and D, time course experiments demonstrating CNT’s to also enhance sCpG^5.5 uptake by GL261 gliomas, but not as efficiently as in BMM. Data is representative of three separate experiments. (n=3 samples /point, ±SEM); *, P<0.05, t-test.

CNTs enhance CpG uptake in vitro. Primary bone marrow-derived monocytes (BMM) from wt or CX3CR1^GFP mice or GL261 gliomas were incubated with PEG-functionalized single-walled CNTs conjugated to Cy5.5-labeled sCpG (CNT-sCpG^5.5, 2.5 µg CNT- 5µg sCpG^5.5/ml) or free sCpG^5.5 (5µg/ml) and sCpG^5.5 uptake was visualized by fluorescent microscopy and quantified by flow cytometry. A, dot plot demonstrating CNT-sCpG uptake by BMM to be more efficient than free sCpG^5.5. B, CpG^5.5 signal was stronger in BMM.gfp cells incubated with CNT-sCpG and was mostly confined to cytoplasmic compartments. C, histogram and D, time course experiments demonstrating CNT’s to also enhance sCpG^5.5 uptake by GL261 gliomas, but not as efficiently as in BMM. Data is representative of three separate experiments. (n=3 samples /point, ±SEM); *, P<0.05, t-test.