CNT-sCpG eradicates established gliomas. Mice bearing four day-old i.c. GL261.luc gliomas (n=6) were given a single i.t. injection of PBS (control), free sCpG (5µg/10µl PBS), blank CNT (2.5 µg) mixed with free sCpG (CNT + sCpG; 5µg CpG/10µl PBS), or sCpG conjugated to CNT (CNT-sCpG; 2.5 µg CNT-5 µg sCpG/10 µl PBS). A, Intracranial tumor burden was assessed by biophotonic imaging. B, Kaplan-Meier analysis demonstrates improved survival of mice treated with a single injection of CNT-sCpG.