Table 1.

Kluyveromyces lactis putative pfam00026 aspartyl proteases

GenBank™accession	Locus tag	Protein length (a.a.)	SP cleavage site*	GPI omega site†	Closest S. cerevisiae homolog	blastpe-value‡
XP_454126	KLLA0E03938g	589	Ala-18	Gly-562	Yps1p	7.5 e-122
XP_456066	KLLA0F22088g	558	Ala-19	ND	Yps7p	8.8 e-80
XP_453761	KLLA0D15917g	511	Cys-18	Gly-490	Bar1p	1.2 e-84
XP_453326	KLLA0D05929g	409	Ala-25	ND	Pep4p	3.6 e-161
XP_453136	KLLA0D01507g	515	Ala-29	ND	Yps6p	3.6 e-11

^*Putative signal peptide cleavage sites were predicted using signalp 3.0 (Bendtsen et al., 2004).

^†Putative glycosylphosphatidylinositol (GPI) anchor attachment (omega) sites were predicted using the big-pi predictor (Eisenhaber et al., 1999).

^‡blastp searches were performed at the SGD website (http://yeastgenome.org). The presented e-values reflect homology to the closest Saccharomyces cerevisiae protein sequence.

ND, not defined.