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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Cancer Res. 2011 Feb 8;71(4):1244–1252. doi: 10.1158/0008-5472.CAN-10-2360

Figure 1.

Figure 1

Uncoupling of the HIF-1α–c-Myc and the HIF-1α–ARNT pathways. A, a schematic representation of HIF-1α with functional domains as indicated. bHLH, basic helix-loop-helix; PAS, Per-ARNT-Sim; ODD, oxygen-dependent degradation; TA, transactivation. The HIF-1α–ARNT and the HIF-1α–c-Myc pathways were inactivated (–) individually by site-directed mutagenesis to create the mutants as indicated. B, transduced U-2 OS cells expressing HIF-1α variants as specified were assayed for target gene expression of the HIF-1α–ARNT pathway (left) and the HIF-1α–c-Myc pathway (right) by real-time PCR. Results are representative of two independent experiments in triplicate and presented as mean ± SD.