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. Author manuscript; available in PMC: 2012 Feb 15.
Published in final edited form as: Cancer Res. 2011 Feb 8;71(4):1244–1252. doi: 10.1158/0008-5472.CAN-10-2360

Figure 3.

Figure 3

Requirement of the HIF-1α–c-Myc pathway for malignant progression and tumorigenicity. A–D, transduced U-2 OS cells as indicated were assayed for anchorage-independent growth (A), Matrigel invasion (B), and tumorigenicity with subcutaneous xenograft in CD-1 nude mice (C). The anchorage-independent colonies and Matrigel invading cells were quantified and presented as mean ± SEM. The injection sites are marked by arrowheads. Summarized result is in (D) with a ratio of 5 mice per group. E, hematoxylin–eosin staining of tumor specimens (T) shows hemorrhagic necrosis (N) and invasion of dermal layers (D) and skeletal muscles (M). Two representative images with 200 × magnification are shown.