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Ex vivo characterization of tumor specimens derived from HIF1α(PP) and HIF1α(PP)+RFC cells. A, total RNA was extracted from the tumors and gene expression as indicated was quantified by real-time PCR in reference to the parental U-2 OS cells. Results are in triplicate and presented as mean ± SD. B, genomic DNA was isolated from the tumors and subjected to microsatellite instability analyses with the 5-marker panel as indicated.
