Fig. 3.

Clusterin expression is increased with HDACI treatment. a NB N-type (IMR32, SH-SY5Y, and GOTO) and S-type (SH-EP1, SK-N-AS, and LA1-5S) cell lines were treated with 1 μM TSA for 24 h before immunoblotting with anti-CLU antibody. β-Tubulin was used as a loading control. b SH-SY5Y and SH-EP1 cell lines were treated with 1 μM TSA and cell lysates were collected at different time points (4, 8,16, 20, and 24 h), and the expression of CLU was analyzed by immunoblotting. c mRNA level CLU is present in S-type SHEP1 cells whereas it is induced after 1 μM TSA treatment in N-type SH-SY5Y cells both after 16 and 24 h. d SH-SY5Y cells and SH-EP1 cell lines were treated with 1 μM of TSA, SAHA, or MS-275 for 24 h, and the expression of CLU was analyzed by immunoblotting