Fig. 5.

Knocking down CLU sensitizes NB cells to TSA-induced cell death. a Various amounts of CLU expression vectors (as indicated) were transfected into SH-SY5Y cells. The cells were treated with different concentration of TSA for 24 h as shown. Cell viability is determined by MTT assay. Results are expressed as the percentage of viable cells compared with vehicle-treated controls (mean ± SD; n = 3). b Stable SH-SY5Y cell line expressing full-length CLU or vector were treated with TSA (1 μM) for 0, 4, 8, and 16 h as indicated. Mitochondria were isolated; Bax level was determined by immunoblotting. VDCA1 is used as the loading control for mitochondria fraction. c SH-SY5Y cells or SH-EP1 cells were transfected with scrambled siRNA or CLU-specific siRNA. Forty-eight hours after transfection, cells were treated with TSA (1 μM, 24 h). Cells in mock sample received no transfection. Immunoblot shows effective knockdown of CLU. d SH-SY5Y cells or SH-EP1 cells were transfected with CLU-specific siRNA as shown in (c). The cells were treated with varying concentrations of TSA for 24 h as shown. Cell viability was determined by MTT assay. Results are expressed as the percentage of viable cells compared with vehicle-treated controls (mean ± SD; n = 3)