Fig. 6.

CLU does not affect Ku70 acetylation or Ku70–Bax binding in NB cells. a Stable SH-SY5Y cell line expressing full-length CLU or vector were treated with TSA (1 μM, 24 h) and cytoplasmic extracts were immunoprecipitated using Bax-specific antibodies or normal rabbit serum (NRS) as control. The immunoprecipitates were separated by SDS–PAGE and probed with Ku70, CLU, or Bax antibodies. b SH-SY5Y cells were treated with vehicle or TSA (1 μM, 24 h). CLU was immunoprecipitated using CLU-specific antibodies or normal mouse serum (NMS). The immunoprecipitates were separated by SDS–PAGE and probed with Ku70 or CLU antibodies. c Stable SH-SY5Y cells expressing full-length CLU or vector were treated with TSA (1 μM, 24 h). Cytoplasmic extracts were immunoprecipitated using an anti-acetyl-lysine antibody (K103). The immunoblot was probed with Ku70 as shown. d CLU siRNA or scramble siRNA-treated SH-SY5Y cells were treated with TSA (1 μM, 24 h). Cytoplasmic extracts were immunoprecipitated using anti-acetyl-lysine antibody (K103). The immunoblot was probed with Ku70 antibody