Fig. 1.
Immortalized cell line produces more Aβ peptides than primary neurons in vitro. a The H4 neuroglioma cell line overexpressing human APPK595N/M596L was assessed for ectopic Aβ production by an immunosorbent assay. The H4 cell line generated more Aβ38, Aβ40, and Aβ42 peptides in a 24-h time period than primary neurons. Aβ40 was the predominant Aβ peptide. b Supernatants of primary telencephalic chicken neurons were examined for endogenous Aβ production by an immunosorbent assay. Primary neurons secreted detectable amounts of Aβ38, Aβ40, and Aβ42 peptides in a 24-h time period. Aβ40 was the predominant Aβ peptide. Graphs represent the individual Aβ content as mean ± standard error of mean in nanogram per milliliter