Fig. 4.
The γ-secretase inhibitor DAPT reduces Aβ formation in primary and immortalized cells. H4 cells overexpressing human APPK595N/M596L and primary telencephalic chicken neurons were treated with different concentrations of the γ-secretase inhibitor DAPT for 24 h. Aβ content of cell supernatants was determined by an immunosorbent assay. a In the H4 cell line, Aβ38, Aβ40, and Aβ42 levels started to be reduced at a concentration of 50 nM DAPT. b In primary telencephalic neurons, Aβ peptides started to diminish at a concentration of 100 nM DAPT. Graphs represent the individual Aβ content in percent of vehicle-treated cultures as mean ± standard error of mean