Fig. 7.
Calpain inhibition increases Aβ42 formation in primary but not immortalized cells. H4 cells overexpressing human APPK595N/M596L and primary telencephalic chicken neurons were treated with different concentrations of calpeptin for 24 h. Aβ content of cell supernatants was determined by an immunosorbent assay. a No modulation of Aβ42 levels was observed in H4 cells. b In contrast, Aβ42 content was increased at 10 μM in primary neuronal cultures. Graphs represent the individual Aβ content in percent of vehicle-treated cultures as mean ± standard error of mean