Table 1.

Primers used to construct recombinant proteins

Primer type	Primer sequence
Mutagenesis
−9 (1248 stop)	F 5′-GGGGCCACTTCCTAAATCAACCCTGCCGTGG-3′
	R 5′-CCACGGCAGGGTTGATTTAGGAAGTGGCCCC-3′
−10 (1247 stop)	F 5′-CAGGGGCCACTTAACCCATCAACCCTGCCG-3′
	R 5′-CGGCAGGGTTGATGGGTTAAGTGGCCCCTG-3′
−20 (1238 stop)	F 5′-AAGAAGGAGAAGGAGGCGTGAGGGGGCAATGA-3′
	R 5′-TCATTGCCCCCTCACGCCTCCTTCTCCTTCTT-3′
−28 (1229 stop)	F 5′-TTGGCCAGTACTAAGGCAAGAAGGAGAAGGA-3′
	R 5′-TCCTTCTCCTTCTTGCCTTAGTACTGGCCAA-3′
P1249A	F 5′-CAGGGGCCACTTCCGCCATCAACCCTGCCG-3′
	R 5′-CGGCAGGGTTGATGGCGGAAGTGGCCCCTG-3′
N1251A	F 5′-GCCACTTCCCCCATCGCCCCTGCCGTGGCCC-3′
	R 5′-GGGCCACGGCAGGGGCGATGGGGGAAGTGGC-3′
N1251D	F 5′-ACTTCCCCCATCGACCCTGCCGTGGCCC-3′
	R 5′-GGGCCACGGCAGGGTCGATGGGGGAAGT-3′
P1252A	F 5′-GCCACTTCCCCCATCAACGCTGCCGTGGCCCTA-3′
	R 5′-TAGGGCCACGGCAGCGTTGATGGGGGAAGTGGC-3′
E1257A	F 5′-GCCGTGGCCCTAGCATAGGAATTCCCG-3′
	R 5′-CGGGAATTCCTATGCTAGGGCCACGGC-3′
Mini-Exon
1238–1257	F 5′-AATTCGCAGGGGGCAATGACAGCTCAGGGGCC
	ACTTCCCCCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGGG
	GAAGTGGCCCCTGAGCTGTCATTGCCCCCTGCG-3′
1249–1257	F 5′-AATTCCCCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGGG-3′
1238–1257 T1247A	F 5′-AATTCGCAGGGGGCAATGACAGCTCAGGGGCCG
	CTTCCCCCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGGG
	GAAGCGGCCCCTGAGCTGTCATTGCCCCCTGCG-3′
1238–1257 S1248A	F 5′-AATTCGCAGGGGGCAATGACAGCTCAGGGGCCA
	CTGCCCCCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGGG
	GCAGTGGCCCCTGAGCTGTCATTGCCCCCTGCG-3′
1238–1257 TS1247/48AA	F 5′-AATTCGGCAATGACAGCTCAGGGGCCGCTGCCC
	CCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGGG
	GCAGCGGCCCCTGAGCTGTCATTGCCG-3′
1238–1257 TS1247/48EE	F 5′-AATTCGGCAATGACAGCTCAGGGGCCGAAGAAC
	CCATCAACCCTGCCGTGGCCCTAGAAG-3′
	R 5′-AATTCTTCTAGGGCCACGGCAGGGTTGATGGG
	TTCTTCGGCCCCTGAGCTGTCATTGCCG-3′