Fig. 2.
Comparison of TNF-induced adhesion molecule upregulation between ECUM and ECCM of subcutaneous fat origin. Cells were seeded in a 96-well plate and stimulated by increasing concentrations of TNF. ECUM and ECCM were then fixed and the expression of ICAM-1, VCAM-1 and CD61 was measured by cell-based ELISA (A, B and C respectively). Results are expressed as mean ± SD of two experiments (Dunn's test, *P < 0.05, **P < 0.01 and ***P < 0.001). As a control, each experiment was confirmed by flow cytometry (D–F). The results of one representative reading obtained with the greatest concentration of TNF are shown here.