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Quantification of TNF-induced endothelial MP release by subcutaneous fat-derived ECUM and ECCM. Cells were cultured and left resting or stimulated with TNF for 6 h before analysis. MP production was quantified by flow cytometry for each condition. Results (two determinations in three experiments) are expressed in numbers of MP labelled with annexin V-FITC, extracted from culture supernatants of 1000 EC (Dunn's test, **P < 0.01).
