Fig. 4.
Comparison of TNF-induced production of MCP-1, RANTES, IL-6 and cleaved Caspase-3 between ECUM and ECCM. Subcutaneous fat-derived EC from both patient categories were stimulated with different doses of TNF. In the case of MCP-1, RANTES and IL-6, supernatants were collected after 12 h of incubation and concentrations were measured by ELISA. For activated Caspase-3, supernatants and cell extracts were pooled before analysis by ELISA. Results are expressed as mean ± SD of three determinations in two experiments (Dunn's test, *P < 0.05 and ***P < 0.001).