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Schematic representation of needle aspiration biopsy. The diagram shows the technique used to isolate subcutaneous fat-derived fat EC by inserting a hollow bore ‘menghini’ needle in the upper external thigh of patients with malaria (UM and CM) after application of a local anaesthetic for 2 h. A strong manual aspiration force represented by the arrow allowed the sampling of approximately 5 mm3 of adipose tissue, from which EC were isolated, selected and cultured before analysis.
