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. 2011 Jan 21;30(4):706–718. doi: 10.1038/emboj.2010.355

Figure 6.

Figure 6

LEV-10 and LEV-9 are not properly localized at the neuromuscular junctions of most oig-4 mutants. (A) Immunostaining of LEV-10 at the dorsal cord of WT and oig-4 mutants. (B) Detection of the knocked-in T7-LEV-9 protein using anti-T7 immunostaining. Scale bar=10 μm. (C) Western blot analysis of LEV-10 expression normalized to total protein content. (Percentage of wild-type levels in the oig-4(kr39) and oig-4(kr193) was 87±7% (n=4) and 114±24% (n=4) respectively, mean±s.d.) Bar indicates the 100 kDa marker. (D) The T7-LEV-9 protein immunoprecipitated from total worm extracts is weakly detected in oig-4 mutants. (E) Weak L-AChR clusters can be detected by immunostaining in rare oig-4(kr39) mutant animals (v) and colocalize with remaining LEV-9-T7 clusters (iv–vi). (F) The weak clusters of T7-LEV-9 infrequently detected in oig-4(kr39) mutants (v), colocalize with remaining staining of the LEV-10 protein (iv–vi). Scale bar=10 μm.