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. 2011 Jan 21;30(4):706–718. doi: 10.1038/emboj.2010.355

Figure 7.

Figure 7

The synaptic localization of GFP-OIG-4 requires L-AChRs while LEV-10 and LEV-9 are partially dispensable. (A) GFP-OIG-4 is no longer clustered in the unc-29(x29) null mutant background (iv). However, small clusters of GFP-OIG-4 can be detected in lev-10(kr26) and lev-9(ox177) null mutants. Pre-synaptic VAChT is labelled using anti-UNC-17 antibodies. Scale bar=10 μm. (B) Western blot analysis of GFP-OIG-4 expression in the unc-29 mutant background (percentage of the wild-type GFP-OIG-4 levels in the unc-29 mutant background was 78.3±10.8% (mean±s.d., n=3)). (C) GFP-OIG-4 expressed in muscle cells under the control of the Pmyo-3 promoter is still secreted and accumulates in coelomocytes of WT and unc-29 null mutants. A wild type-like GFP-OIG-4 translational fusion expressed by the muscle promoter Pmyo-3 in transgenic oig-4;unc-29 mutants is detected in coelomocytes. Coelomocytes (dotted circle) are visualized by Nomarski (i, iii) and epifluorescence microscopy (ii, iv) in unc-29(+) (i, ii) and unc-29 null mutant background (iii, iv). Asterisk indicates autofluorescence of intestine. Scale bar=10 μm.