Figure 7.
Dynein numbers and EE escape rate at MT plus-ends. (A) Pseudo-coloured image of an apical dynein comet (strain AB33G3Dyn2). The bar represents one micrometer. (B) Graph showing intensity profile along the length of the dynein comet. Values are mean±standard error of the mean of linescan analysis of 20 apical dynein comets. Note that the distribution of the dynein along the length of the MT is independent of the overall intensity of the comet. (C) Kymograph showing the arrival and turning of photoactivated paRab5a-carrying EEs (green lines) and dynein heavy chain fused to a triple mCherry tag (red). Note that only one of the EEs arrived at the very end of the MT, where dynein concentration is highest. (D) Turning of arriving EEs within the dynein comet. Note that the MT plus-end was defined by the maximum intensity of the dynein signal in the comet. (E) Bar chart showing the behaviour of EEs in control and cells expressing the peptide Peb1c. Note that Peb1c reduces the average number of dyneins to ∼20, which results in 9.22% loss of EEs at the MT end. Triple asterisks indicate significant difference to control at P<0.0001.