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. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Shock. 2011 Mar;35(3):282–288. doi: 10.1097/SHK.0b013e3181fddd82

Decreased NO Synthesis was Associated with Increased ADMA Concentrations and Decreased DDAH-2 Expression. Two (n=6) and three (n=6) weeks after 20% TBSA full thickness burn and a smoke inhalation injury, sheep were killed and plasma was collected over time until two or three weeks before and after the injury to measure (A) the products of nitric oxide, nitrates and nitrites, and (B) arginine concentrations. (C) Lung homogenate was used to measure ADMA concentrations using LC-MS, and to measure (D) lung DDAH-2 expression and (E) lung DDAH-1 expression. The increase in ADMA is associated with the decrease in DDAH-2 and the decrease in NOx. (F) Plasma was collected over time until two or three weeks before and after the injury to measure citrulline concentrations. Results were compared to uninjured sham animals that were not injured but treated in the same manner. Equality of protein loading was confirmed by the expression of B-actin, and each band was quantified by densitometric analysis. Data are shown as means ± SEM. †P < 0.05 versus uninjured animals, §P < 0.05 versus baseline data, ∞P < 0.05 versus injured animals sacrificed after 2 wks.

Decreased NO Synthesis was Associated with Increased ADMA Concentrations and Decreased DDAH-2 Expression. Two (n=6) and three (n=6) weeks after 20% TBSA full thickness burn and a smoke inhalation injury, sheep were killed and plasma was collected over time until two or three weeks before and after the injury to measure (A) the products of nitric oxide, nitrates and nitrites, and (B) arginine concentrations. (C) Lung homogenate was used to measure ADMA concentrations using LC-MS, and to measure (D) lung DDAH-2 expression and (E) lung DDAH-1 expression. The increase in ADMA is associated with the decrease in DDAH-2 and the decrease in NOx. (F) Plasma was collected over time until two or three weeks before and after the injury to measure citrulline concentrations. Results were compared to uninjured sham animals that were not injured but treated in the same manner. Equality of protein loading was confirmed by the expression of B-actin, and each band was quantified by densitometric analysis. Data are shown as means ± SEM. †P < 0.05 versus uninjured animals, §P < 0.05 versus baseline data, ∞P < 0.05 versus injured animals sacrificed after 2 wks.